The Molecular Biology of Plant Cells

The University of California Press is making available this somewhat vintage botany textbook – it was edited in 1977 by Harry Smith and contains materials written by educators and researchers from the USA, England, Australia and Canada. It’s my hope that despite its age, it will provide some great instruction for students looking for help with the topics covered.

The Molecular Biology of Plant Cells textbook was written for senior undergraduate and post-graduate students studying biology, biochemistry, botany, molecular biology and agricultural science. It does cover basic cellular physiology, biochemistry and the genetics of plant cells. However, it does not cover metabolic pathways or the physiology and biochemistry of the intact plant.

Table of Contents

Section One – Plant Cell Structure and Function

Chapter 1 — Plant Cell Walls

1.1 — Introduction

1.2 — The Molecular Structure of Plant Cell Walls

1.3 — Interconnections between Cell Wall Components

1.4 — The Universality of Plant Cell Wall Structures

1.5 — Cell Wall Plastics

1.6 — Biosynthesis of Plant Cell Walls

1.7 — Cell Wall Ultrastructure

1.8 — Hormonal Control of Cell Wall Biosynthesis and Differentiation

1.9 — The Role of the Plant Cell Wall in Interactions with Other Organisms

Chapter 2 — Membrane Structure and Transport

2.1 — Introduction

2.2 — Chemical Composition of Membranes

2.3 — Membrane Structure

2.4 — Transport of Substances Across Membranes

2.5 — Correlation of Structure and Function

Chapter 3 — Chloroplasts—Structure and Development

3.1 — Introduction

3.2 — Chloroplast Structure

3.3 — Isolation of Chloroplasts

3.4 — Chloroplast Development

3.5 — Summary

Chapter 4 — Chloroplasts—Structure and Photosynthesis

4.1 — Introduction

4.2 — Thylakoid Structure

4.3 — Thylakoid Composition

4.4 — Photosynthesis

4.5 — Subchloroplast Fragments and the Fractionation of the Photosystems

4.6 — Thylakoid Structure in Relation to the Photosystems

4.7 — Assembly of the Thylakoid Membrane

4.8 — Relationship of Chloroplast to Cytoplasm

Chapter 5 — Plant Mitochondria

5.1 — Introduction

5.2 — Morphology

5.3 — Isolation and Purification

5.4 — Mitochondrial Membranes

5.5 — Enzymes

5.6 — Mitochondrial Electron Transport

5.7 — Energy Linked Reactions of Mitochondria

Chapter 6 — Microbodies

6.1 — Introduction

6.2 — Structure and Occurrence

6.3 — Isolation

6.4 — Glyoxysomes

6.5 — Peroxisomes

6.6 — Ontogeny and Turnover of Microbodies

6.7 — Algal Microbodies

Chapter 7 — Microtubules

7.1 — Introduction

7.2 — Biochemical Studies

7.3 — Biological Studies

7.4 — Concluding Remarks

Chapter 8 — The Endomembrane System and the Integration of Cellular Activities

8.1 — Introduction

8.2 — Techniques

8.3 — Composition and Characteristics of the Membrane Types

8.4 — Functional Relationships between Membranes

8.5 — Conclusions

Section Two – Gene Expression and Its Regulation in Plant Cells

Chapter 9 — The Nucleus and the Organization and Transcription of Nuclear DNA

9.1 — Introduction

9.2 — Nuclear Structure and Composition

9.3 — Nuclear DNA Content

9.4 — Fractionation and Properties of DNA

9.5 — The Genes for Ribosomal-RNA

9.6 — Organization and Function of the Genome

9.7 — RNA Synthesis

9.8 — Nucleic Acid-Protein Interactions

9.9 — Conclusions

Chapter 10 — Protein Synthesis in the Cytoplasm

10.1 — Introduction

10.2 — Polysomes

10.3 — The Biochemical Mechanism of Protein Synthesis

10.4 — Protein Synthesis in vivo

Chapter 11 — The Genetic Information of Organelles and Its Expression

11.1 — Introduction: The Concept of Organelle Autonomy

11.2 — Chloroplast Autonomy

11.3 — Mitochondrial Autonomy

Chapter 12- Regulation of Enzyme Levels and Activity

12.1 – Introduction

12.2 – The Identification of Control Points

12.3 – Control Mechanisms

12.4 – Control Mechanisms Involving Changes in the Amount of Enzyme

12.5 – Final Comments

Chapter 13- Hormone Action

13.1 – Introduction

13.2 – Auxin Actions

13.3 – Gibberellin Action

13.4 – Action of Cytokinins

13.5 – Actions of Ethylene

13.6 – Actions of Abscisic Acid

Chapter 14 – Phytochrome Action

14.1 – Introduction

14.2 – Phytochrome Dogma

14.3 – The Phytochrome Molecule

14.4 – Phytochrome Physiology

14.5 – Mechanism of Action

Section Three – The Manipulation of Plant Cells

Chapter 15- Plant Cell Culture

15.1 – Introduction

15.2 – Changes in Growth and Metabolism of Plant Cells in Batch Culture-Cytodifferentiation

15.3 – Steady States of Growth and Metabolism of Plant Cells in Continuous Culture

15.4 – Synchronous Cell Cultures—Study of the Cell Cycle

15.5 – Morphogenesis in Cell Cultures—Concepts of Totipotency and Determination

15.6 – Pollen Grains as Isolated Embryogenic Cells and as a Source of Haploid Cell Lines for Mutagenesis

Chapter 16 – The Physiology of Isolated Plant Protoplasts

16.1 – Introduction

16.2 – Isolation and Culture of Protoplasts

16.3 – Protoplasts and Auxin Responses

16.4 – Uptake Properties of Protoplasts

16.5 – Somatic Hybridization of Plants

Chapter 17 – Genetic Variation in Cultured Plant Cells

17.1 – Introduction

17.2 – Induced Variation and Selection

17.3 – Introduced Variation—Gene Transfer

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The Molecular Biology of Plant Cells